PAX6 Rekombinanter Antikörper

PAX6 Rekombinant Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241521G1

Kat-Nr. : 84345-5-PBS

Synonyme

241521G1, paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6

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  WB analysis using 84345-5-RR (same clone as 84345-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84345-5-RR (PAX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84345-5-RR (PAX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 84345-5-RR (same clone as 84345-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PAX6 antibody (84345-5-RR, Clone: 241521G1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 84345-5-RR (same clone as 84345-5-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug PAX6 Recombinant antibody (84345-5-RR, Clone:241521G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84345-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84345-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84345-5-RR against Human PAX6 were performed. The affinity constant is 40.0 pM.

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Geprüfte Anwendungen

Produktinformation

84345-5-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA PAX6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PAX6 fusion protein Ag2984
• Vollständiger Name: paired box 6
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC011953
• Gene symbol: PAX6
• Gene ID (NCBI): 5080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.