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  • KD/KO Validated

PAX8 Polyklonaler Antikörper

PAX8 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(544)

Kat-Nr. : 10336-1-AP

Synonyme

PAX-8, paired box 8, Paired box protein Pax 8, Paired box protein Pax-8, PAX 8



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSKOV-3-Zellen, HEK-293-Zellen, NIH/3T3-Zellen, Raji-Zellen
Erfolgreiche IPSKOV-3-Zellen, HeLa-Zellen
Erfolgreiche Detektion in IHChuman ovary cancer tissue, humanes Nierenzellkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe, Mausnierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMausnierengewebe
Erfolgreiche Detektion in IF/ICCSKOV-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:800-1:3200
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10336-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA PAX8 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Kaninchen, Maus, Ratte, Rind, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name paired box 8
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48 kDa, 58 kDa
GenBank-ZugangsnummerBC001060
Gene symbol PAX8
Gene ID (NCBI) 7849
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PAX8 antibody 10336-1-APProtokoll herunterladen
IHC protocol for PAX8 antibody 10336-1-APProtokoll herunterladenl
IF protocol for PAX8 antibody 10336-1-APProtokoll herunterladen
IP protocol for PAX8 antibody 10336-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,CoIP

Nature

The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.

Authors - Saroor A Patel
  • KD Validated
humanIHC

Nat Biotechnol

Tubuloids derived from human adult kidney and urine for personalized disease modeling.

Authors - Frans Schutgens
humanIHC

Nat Med

Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice.

Authors - Sabine Harlander
mouse,humanWB,IHC

Cancer Discov

A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma.

Authors - Phillip M Rappold
humanIHC

Cell Stem Cell

Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer.

Authors - Kadi Lõhmussaar
humanIF

Cell Stem Cell

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.

Authors - Jian Hui Low

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kenzo (Verified Customer) (03-28-2024)

Worked well for immunolabeling on mouse retina tissue sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Retina
FH

Eunyoung (Verified Customer) (02-28-2020)

This antibody works well on the mouse ovarian cancer tissue

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovary /fallopian tissue
PAX8 Antibody Immunohistochemistry, validation (1:1000 dilution) in Ovary /fallopian tissue (Cat no:10336-1-AP)
FH

shuang (Verified Customer) (02-18-2020)

This Pax8 antibody works great for both IHC and IF staining on mouse Fallopian tube/uterus organoids and tumors, Highly recommend!

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse Fallopian tube/ Fallopian tube organoids
FH

Adam (Verified Customer) (05-30-2019)

Antibody works great for our applications (IHC on FFPE tissues). Only comment is that it cane sometimes have some background staining, particularly in some mouse tissues such as liver.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
FH

Huai-Chin (Verified Customer) (04-15-2019)

So far only try the antibody for IHC on mouse tissue. Works pretty good. Would like to try it on human tissue/cell lines.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Ovary/Oviduct
FH

Paulo (Verified Customer) (03-03-2019)

Used to perform ChIP-seq in ccRCC cell lines. 17.5ug of antibody used in 100million cells. IP was validated by WB. Sequencing detected PAX8 binding genome wide. Motif analysis identified the consensus PAX8 binding motif in approximately 90% of detected peaks.Would be great if Proteintech would produce a more concentrated version of this antibody for ChIP-seq.

  • Applications: ChIP,
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