PBLD Monoklonaler Antikörper

PBLD Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1G11D4

Kat-Nr. : 68317-1-Ig

Synonyme

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

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  WB analysis of pig liver using 68317-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 68317-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 68317-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, Hausschwein-Nierengewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

68317-1-Ig bindet in WB, IF/ICC, ELISA PBLD und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PBLD fusion protein Ag23827
• Vollständiger Name: phenazine biosynthesis-like protein domain containing
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC009738
• Gene symbol: PBLD
• Gene ID (NCBI): 64081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Discovery of vitexin as a novel VDR agonist that mitigates the transition from chronic intestinal inflammation to colorectal cancer; Authors - Yonger Chen
human,mouse	WB	Nat Commun; Phenazine biosynthesis-like domain-containing protein (PBLD) and Cedrelone promote antiviral immune response by activating NF-ĸB; Authors - Peili Hou ; KO Validated