PBLD Monoklonaler Antikörper

PBLD Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G11D4

Kat-Nr. : 68317-1-PBS

Synonyme

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

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  WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68317-1-PBS bindet in WB, IF/ICC, Indirect ELISA PBLD und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PBLD fusion protein Ag23827
• Vollständiger Name: phenazine biosynthesis-like protein domain containing
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC009738
• Gene symbol: PBLD
• Gene ID (NCBI): 64081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)