PBRM1 Rekombinanter Antikörper

PBRM1 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240655H5

Kat-Nr. : 83677-2-PBS

Synonyme

240655H5, BAF180, BRG1-associated factor 180, hPB1, PB1

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  WB analysis using 83677-2-RR (same clone as 83677-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83677-2-RR (PBRM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83677-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83677-2-RR (same clone as 83677-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBRM1 antibody (83677-2-RR, Clone: 240655H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83677-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83677-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83677-2-PBS against Human PBRM1 was performed. The affinity constant is 3.68 nM.

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  Affinity and Kinetic Characterization of 83677-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83677-2-RR against Human PBRM1 were performed. The affinity constant is 2.34 nM.

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Geprüfte Anwendungen

Produktinformation

83677-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA PBRM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PBRM1 fusion protein Ag33623
• Vollständiger Name: polybromo 1
• Berechnetes Molekulargewicht: 180 kDa
• Beobachtetes Molekulargewicht: 180-195 kDa
• GenBank-Zugangsnummer: BC015323
• Gene symbol: PBRM1
• Gene ID (NCBI): 55193
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.