PBX1 Polyklonaler Antikörper

PBX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 18204-1-AP

Synonyme

PRL, Pre-B-cell leukemia transcription factor 1, pre B cell leukemia homeobox 1, Homeobox protein PRL, Homeobox protein PBX1

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  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18204-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18204-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 18204-1-AP

  IP result of anti-PBX1 (IP:18204-1-AP, 4ug; Detection:18204-1-AP 1:500) with A2780 cells lysate 1000ug.

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  IHC staining of mouse kidney using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 18204-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18204-1-AP

  Immunofluorescent analysis of HeLa cells using 18204-1-AP (PBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HeLa-Zellen
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Zervixkarzinomgewebe, Maushirngewebe, Mausnierengewebe, Maus-Pankreasgewebe, Rattenhirngewebe, Rattennierengewebe, Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

18204-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA PBX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBX1 fusion protein Ag12817
• Vollständiger Name: pre-B-cell leukemia homeobox 1
• Berechnetes Molekulargewicht: 430 aa, 47 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC101578
• Gene symbol: PBX1
• Gene ID (NCBI): 5087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b) and molecular weight of PBX1 is 47 kDa and 38 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,rat	WB,IF	Int J Oral Sci; Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis; Authors - Xiao Yang
mouse	WB	J Neurochem; Decreased levels of constitutive proteasomes in experimental autoimmune encephalomyelitis may be caused by a combination of subunit displacement and reduced Nfe2l1 expression.; Authors - Kara L Shanley
mouse	WB	Neuroscience; Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.; Authors - Zhengfang Lu
human	WB	Cell Death Dis; M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops; Authors - Hongda Lin
mouse	ChIP	Cell Mol Life Sci; Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility; Authors - Fei-Chen Wang