PBX1 Rekombinanter Antikörper

PBX1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251373B4

Kat-Nr. : 86504-3-RR

Synonyme

Homeobox protein PBX1, Homeobox protein PRL, pre B cell leukemia homeobox 1, Pre-B-cell leukemia transcription factor 1, PRL

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  WB analysis of HeLa using 86504-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86504-3-RR (PBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86504-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86504-3-RR (PBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 86504-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 86504-3-RR (PBX1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86504-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX1 antibody (86504-3-RR, Clone: 251373B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86504-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86504-3-RR against Human PBX1 were performed. The affinity constant is 0.342 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86504-3-RR bindet in WB, IHC, IF/ICC, ELISA PBX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PBX1 fusion protein Ag12817
• Vollständiger Name: pre-B-cell leukemia homeobox 1
• Berechnetes Molekulargewicht: 430 aa, 47 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 47 kDa
• GenBank-Zugangsnummer: BC101578
• Gene symbol: PBX1
• Gene ID (NCBI): 5087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b: MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b), and molecular weight of PBX1 is 47 kDa and 38 kDa.