PBX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PBX3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, chIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 12571-1-AP

Synonyme

Homeobox protein PBX3, pre B cell leukemia homeobox 3, Pre-B-cell leukemia transcription factor 3

---

• 

  WB analysis of human lung using 12571-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 12571-1-AP

  IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug.

• 

  IHC staining of human lung cancer using 12571-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX3 antibody (12571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Produktinformation

12571-1-AP bindet in WB, IHC, IF/ICC, IP, chIP, RIP, ELISA PBX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBX3 fusion protein Ag3239
• Vollständiger Name: pre-B-cell leukemia homeobox 3
• Berechnetes Molekulargewicht: 434 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC016977
• Gene symbol: PBX3
• Gene ID (NCBI): 5090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBX3 is a homeodomain-containing transcription factor of the pre-B cell leukemia (PBX) family, members of which have extensive roles in early development and some adult processes. It plays crucial roles in embryonic development, organogenesis, and cell differentiation by regulating the expression of downstream target genes. Beyond its physiological functions, PBX3 is increasingly recognized for its significant involvement in oncogenesis. It is frequently overexpressed in various malignancies, including leukemias and solid tumors, where it promotes cell proliferation, inhibits apoptosis, enhances invasion and metastasis, and contributes to chemotherapy resistance.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis.; Authors - Yongyan Wu
human	WB	Haematologica; P2X7 promotes the progression of MLL-AF9 induced acute myeloid leukemia by upregulation of Pbx3.; Authors - Wenli Feng ; KD Validated
mouse,human	IF	Cell Mol Gastroenterol Hepatol; scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.; Authors - Christina M Wright
rat	IF	Free Radic Biol Med; Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.; Authors - Jingjing Zhang
mouse,human	WB,IHC	Int J Biol Sci; USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling.; Authors - Dandan Wang
rat,human	IF	Sci Rep; Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.; Authors - Jingjing Zhang