PBX3 Polyklonaler Antikörper

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• KD/KO Validated

PBX3 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12571-1-AP

Synonyme

Homeobox protein PBX3, PBX3, pre B cell leukemia homeobox 3

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Galerie der Validierungsdaten

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  WB analysis of human lung using 12571-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 12571-1-AP

  IP Result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug.

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  IHC staining of human lung cancer using 12571-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12571-1-AP (PBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lungengewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12571-1-AP bindet in WB, RIP, IP, IHC, IF, chIP, ELISA PBX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBX3 fusion protein Ag3239
• Vollständiger Name: pre-B-cell leukemia homeobox 3
• Berechnetes Molekulargewicht: 434 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC016977
• Gene symbol: PBX3
• Gene ID (NCBI): 5090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBX3, also named as Pre-B-cell leukemia transcription factor 3, is a 434 amino acid protein ,which contains 1 homeobox DNA-binding domain and belongs to the TALE/PBX homeobox family. PBX3 localizes in the nucleus and as a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. PBX3 is ubiquitously expressed in many tissues.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis.; Authors - Yongyan Wu
human	WB	Haematologica; P2X7 promotes the progression of MLL-AF9 induced acute myeloid leukemia by upregulation of Pbx3.; Authors - Wenli Feng ; KD Validated
mouse,human	IF	Cell Mol Gastroenterol Hepatol; scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.; Authors - Christina M Wright
rat	IF	Free Radic Biol Med; Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.; Authors - Jingjing Zhang
human,mouse	WB,IHC	Int J Biol Sci; USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling.; Authors - Dandan Wang
rat,human	IF	Sci Rep; Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.; Authors - Jingjing Zhang