PBX3 Polyklonaler Antikörper

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• KD/KO Validated

PBX3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12571-1-PBS

Synonyme

Homeobox protein PBX3, pre B cell leukemia homeobox 3, Pre-B-cell leukemia transcription factor 3

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  WB analysis of human lung using 12571-1-AP (same clone as 12571-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 12571-1-AP (same clone as 12571-1-PBS)

  IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12571-1-AP (same clone as 12571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12571-1-AP (same clone as 12571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX3 antibody (12571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12571-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PBX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBX3 fusion protein Ag3239
• Vollständiger Name: pre-B-cell leukemia homeobox 3
• Berechnetes Molekulargewicht: 434 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC016977
• Gene symbol: PBX3
• Gene ID (NCBI): 5090
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBX3 is a homeodomain-containing transcription factor of the pre-B cell leukemia (PBX) family, members of which have extensive roles in early development and some adult processes. It plays crucial roles in embryonic development, organogenesis, and cell differentiation by regulating the expression of downstream target genes. Beyond its physiological functions, PBX3 is increasingly recognized for its significant involvement in oncogenesis. It is frequently overexpressed in various malignancies, including leukemias and solid tumors, where it promotes cell proliferation, inhibits apoptosis, enhances invasion and metastasis, and contributes to chemotherapy resistance.