PBX4 Polyklonaler Antikörper

PBX4 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22323-1-AP

Synonyme

Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 22323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22323-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22323-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 22323-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22323-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 22323-1-AP

  IP result of anti-PBX4 (IP:22323-1-AP, 4ug; Detection:22323-1-AP 1:300) with A2780 cells lysate 3600 ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A2780-Zellen, COLO 320-Zellen, Jurkat-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: A2780-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22323-1-AP bindet in WB, IP,ELISA PBX4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBX4 fusion protein Ag17768
• Vollständiger Name: pre-B-cell leukemia homeobox 4
• Berechnetes Molekulargewicht: 374 aa, 41 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC141859
• Gene symbol: PBX4
• Gene ID (NCBI): 80714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pre-B-cell leukemia homeobox 4 (PBX4), also named as Homeobox protein PBX4, is one of the pbx gene encode homeodomain containing transcriptional regulators that interact with other proteins to control embryogenesis and tumorigenesis. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase. PBX4 may form heterodimers with other homeobox genes (hoxb1b or meis3) to specify different developmental fates during vertebrate embryogenesis (PMID:10679934). The trimeric complex containing Pbx4, Meis3, and Hoxb1b was also formed. The calculated molecular weight of PBX4 is about 41 kDa, but the molecular weight of heterodimers (PBX4 and hoxb1b) is about 70 kDa.