PBXIP1 Polyklonaler Antikörper

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• KD/KO Validated

PBXIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 12102-1-AP

Synonyme

Pre-B-cell leukemia transcription factor-interacting protein 1, HPIP, Hematopoietic PBX-interacting protein

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  WB analysis of BxPC-3 using 12102-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12102-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12102-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12102-1-AP

  IP result of anti-PBXIP1 (IP:12102-1-AP, 3ug; Detection:12102-1-AP 1:800) with HeLa cells lysate 3000ug.

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12102-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

12102-1-AP bindet in WB, IHC, IF/ICC, IP, chIP, ELISA PBXIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBXIP1 fusion protein Ag2746
• Vollständiger Name: pre-B-cell leukemia homeobox interacting protein 1
• Berechnetes Molekulargewicht: 731 aa, 81 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC016852
• Gene symbol: PBXIP1
• Gene ID (NCBI): 57326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
human	WB,IF	Cell Death Differ; MDM2 restrains estrogen-mediated AKT activation by promoting TBK1-dependent HPIP degradation.; Authors - K Shostak
	WB	Hum Genet; Human organoids for rapid validation of gene variants linked to cochlear malformations; Authors - Mohammad Faraz Zafeer
human	WB,IHC,IF	Oncogenesis; The interplay between HPIP and casein kinase 1α promotes renal cell carcinoma growth and metastasis via activation of mTOR pathway.; Authors - H Mai ; KD Validated
human	WB,IHC	Cancer Sci; Hematopoietic pre-B cell leukemia transcription factor interacting protein is overexpressed in gastric cancer and promotes gastric cancer cell proliferation, migration, and invasion.; Authors - Yingying Feng ; KD Validated
human, mouse	WB, IHC	Sci Rep; HPIP is upregulated in colorectal cancer and regulates colorectal cancer cell proliferation, apoptosis and invasion.; Authors - Yingying Feng