PBXIP1 Polyklonaler Antikörper

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PBXIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12102-1-PBS

Synonyme

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  WB analysis of BxPC-3 using 12102-1-AP (same clone as 12102-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12102-1-AP (same clone as 12102-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12102-1-AP (PBXIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

  IP result of anti-PBXIP1 (IP:12102-1-AP, 3ug; Detection:12102-1-AP 1:800) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12102-1-AP (same clone as 12102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12102-1-AP (same clone as 12102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12102-1-AP (PBXIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12102-1-AP (same clone as 12102-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12102-1-AP (same clone as 12102-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PBXIP1 antibody (12102-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12102-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12102-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PBXIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBXIP1 fusion protein Ag2746
• Vollständiger Name: pre-B-cell leukemia homeobox interacting protein 1
• Berechnetes Molekulargewicht: 731 aa, 81 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC016852
• Gene symbol: PBXIP1
• Gene ID (NCBI): 57326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).