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Pyruvate Carboxylase Polyklonaler Antikörper
Pyruvate Carboxylase Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16588-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Mauslebergewebe, HepG2-Zellen, Rattenlebergewebe |
Erfolgreiche IP | HepG2-Zellen, Mauslebergewebe |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 38 publications below |
IHC | See 5 publications below |
IF | See 4 publications below |
IP | See 3 publications below |
Produktinformation
16588-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Pyruvate Carboxylase und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Pyruvate Carboxylase fusion protein Ag8857 |
Vollständiger Name | pyruvate carboxylase |
Berechnetes Molekulargewicht | 1178 aa, 130 kDa |
Beobachtetes Molekulargewicht | 125-130 kDa |
GenBank-Zugangsnummer | BC011617 |
Gene symbol | Pyruvate Carboxylase |
Gene ID (NCBI) | 5091 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Pyruvate Carboxylase antibody 16588-1-AP | Protokoll herunterladen |
IHC protocol for Pyruvate Carboxylase antibody 16588-1-AP | Protokoll herunterladenl |
IF protocol for Pyruvate Carboxylase antibody 16588-1-AP | Protokoll herunterladen |
IP protocol for Pyruvate Carboxylase antibody 16588-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Commun Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells | ||
Adv Sci (Weinh) Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway | ||
Nat Commun SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition. | ||
Nat Commun Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise. | ||
Nat Commun The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase. | ||
Cancer Res A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kevin (Verified Customer) (02-10-2022) | This worked as well as other much more expensive antibodies from differenet suppliers
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