PCARE Rekombinanter Antikörper

PCARE Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240352F6

Kat-Nr. : 83364-4-PBS

Synonyme

C2orf71, 240352F6

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  WB analysis using 83364-4-RR (same clone as 83364-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83364-4-RR (PCARE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 83364-4-RR (same clone as 83364-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83364-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83364-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83364-4-RR against Human PCARE were performed. The affinity constant is 3.06 nM.

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Geprüfte Anwendungen

Produktinformation

83364-4-PBS bindet in WB, IHC, IF-P, ELISA PCARE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PCARE fusion protein Ag34774
• Vollständiger Name: chromosome 2 open reading frame 71
• Berechnetes Molekulargewicht: 140 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_001029883
• Gene symbol: PCARE
• Gene ID (NCBI): 388939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).