PCBP3 Polyklonaler Antikörper

PCBP3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16141-1-AP

Synonyme

PCBP3-overlapping transcript 1, PCBP3-overlapping transcript, PCBP3-OT1, PCBP3OT, Alpha-CP3

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  WB analysis using 16141-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16141-1-AP (PCBP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 16141-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PCBP3 antibody (16141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16141-1-AP bindet in WB, IF/ICC, ELISA PCBP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCBP3 fusion protein Ag9202
• Vollständiger Name: poly(rC) binding protein 3
• Berechnetes Molekulargewicht: 313 aa, 33 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012061
• Gene symbol: PCBP3
• Gene ID (NCBI): 54039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.