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PCCB Monoklonaler Antikörper

PCCB Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9A5

Kat-Nr. : 66501-1-PBS

Synonyme

1A9A5, EC:6.4.1.3, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, Propionyl-CoA carboxylase beta chain, mitochondrial

Filter:
  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis of HepG2 using 66501-1-Ig (same clone as 66501-1-PBS)

    WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IF Staining of mouse kidney using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PCCB antibody (66501-1-Ig, Clone: 1A9A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IF Staining of human kidney using 66501-1-Ig (same clone as 66501-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50335-2

    Cytometric bead array standard curve of MP50335-2

    Cytometric bead array standard curve of MP50335-2, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-2-PBS. Detection antibody: 66501-1-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50335-3

    Cytometric bead array standard curve of MP50335-3

    Cytometric bead array standard curve of MP50335-3, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-1-PBS. Detection antibody: 66501-3-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 66501-1-Ig

    Affinity and Kinetic Characterization of 66501-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 66501-1-Ig against Human PCCB was performed. The affinity constant is 2.99 nM.

Geprüfte Anwendungen

Produktinformation

66501-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA PCCB und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PCCB fusion protein Ag1608
Vollständiger Name propionyl Coenzyme A carboxylase, beta polypeptide
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.