PCDH15 Polyklonaler Antikörper

PCDH15 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20719-1-AP

Synonyme

DFNB23, PCDH15, protocadherin 15, USH1F

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  WB analysis using 20719-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20719-1-AP

  mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 20719-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20719-1-AP bindet in WB, IF/ICC, ELISA PCDH15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protocadherin 15
• Berechnetes Molekulargewicht: 216 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 50 kDa
• GenBank-Zugangsnummer: NM_033056
• Gene symbol: PCDH15
• Gene ID (NCBI): 65217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).