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PCDH15 Polyklonaler Antikörper

PCDH15 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20719-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using PCDH15 Polyclonal antibody (20719-1-AP)

    WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCDH15 Polyclonal antibody (20719-1-AP)

    WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PCDH15 Polyclonal antibody (20719-1-AP)

    IF Staining of HEK-293 using 20719-1-AP (same clone as 20719-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20719-1-PBS bindet in WB, IF/ICC, Indirect ELISA PCDH15 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name protocadherin 15
Berechnetes Molekulargewicht 216 kDa
Beobachtetes Molekulargewicht120-160 kDa, 50 kDa
GenBank-ZugangsnummerNM_033056
Gene symbol PCDH15
Gene ID (NCBI) 65217
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).