PCDH21 Rekombinanter Antikörper

PCDH21 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241134E10

Kat-Nr. : 84084-3-RR

Synonyme

PRCAD, Photoreceptor cadherin, KIAA1775, CDHR1, Cadherin-related family member 1

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  WB analysis of Y79 using 84084-3-RR

  Y79 cells were subjected to SDS PAGE followed by western blot with 84084-3-RR (PCDH21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84084-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84084-3-RR against Human PCDH21 were performed. The affinity constant is 0.196 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84084-3-RR bindet in WB, ELISA PCDH21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PCDH21 fusion protein Ag11451
• Vollständiger Name: protocadherin 21
• Berechnetes Molekulargewicht: 745 aa, 82 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC038799
• Gene symbol: PCDH21
• Gene ID (NCBI): 92211
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). PCDH21 (protocadherin 21), also known as CDHR1, is a photoreceptor-specific cadherin protein containing six ECs and an intracellular domain. The mutations of PCDH21 cause recessive retinal degeneration and autosomal recessive cone-rod dystrophy in humans (PMID: 16288196; PMID: 20805371). The full length of the PCDH21 protein is 120 kDa, the N-terminal soluble fragment is 95 kDa, and the C-terminal fragment is 25 kDa (PMID: 35066146; PMID: 18654668; PMID: 33964272).