PCDH24 Polyklonaler Antikörper

PCDH24 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 27103-1-AP

Synonyme

CDHR2, PC LKC, PCDH24, PCLKC, protocadherin 24, Protocadherin LKC

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 27103-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (PCDH24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Dünndarmgewebe, humanes Lebergewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

27103-1-AP bindet in WB, IHC, ELISA PCDH24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDH24 fusion protein Ag25840
• Vollständiger Name: protocadherin 24
• Berechnetes Molekulargewicht: 1310 aa, 142 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC130282
• Gene symbol: PCDH24
• Gene ID (NCBI): 54825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Transl Androl Urol; Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.; Authors - Xuechao Li