PCDHA2 Polyklonaler Antikörper

PCDHA2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10127-2-AP

Synonyme

PCDH alpha 2, PCDH ALPHA2, PCDHA2, protocadherin alpha 2

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Galerie der Validierungsdaten

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  WB analysis using 10127-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10127-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10127-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10127-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10127-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10127-2-AP

  IP Result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Herzgewebe, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Raji-Zellen, Ratten-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10127-2-AP bindet in WB, IP,ELISA PCDHA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDHA2 fusion protein Ag0173
• Vollständiger Name: protocadherin alpha 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa, 80 kDa
• GenBank-Zugangsnummer: BC003126
• Gene symbol: PCDHA2
• Gene ID (NCBI): 56146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).