PCDHA2 Polyklonaler Antikörper

PCDHA2 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10127-2-PBS

Synonyme

---

• 

  WB analysis using 10127-2-AP (same clone as 10127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10127-2-AP (same clone as 10127-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 10127-2-AP (same clone as 10127-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10127-2-AP (same clone as 10127-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  IP result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

10127-2-PBS bindet in WB, IP, Indirect ELISA PCDHA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDHA2 fusion protein Ag0173
• Vollständiger Name: protocadherin alpha 2
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa, 80 kDa
• GenBank-Zugangsnummer: BC003126
• Gene symbol: PCDHA2
• Gene ID (NCBI): 56146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).

{{ptg:RelatedPrimaryAntibodies}}