PCDHA3 Polyklonaler Antikörper

PCDHA3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18803-1-AP

Synonyme

Protocadherin alpha-3, PCDH-alpha-3

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  WB analysis using 18803-1-AP

  Calu-3 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18803-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 18803-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Calu-3 cells, A549-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18803-1-AP bindet in WB, IHC, ELISA PCDHA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protocadherin alpha 3
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC129807
• Gene symbol: PCDHA3
• Gene ID (NCBI): 56145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.