PCDHA9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PCDHA9 Polyklonal Antikörper für IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18075-1-AP

Synonyme

Protocadherin alpha-9, PCDH-alpha-9, KIAA0345

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  IP experiment of mouse brain using 18075-1-AP

  IP result of anti-PCDHA9 (IP:18075-1-AP, 4ug; Detection:18075-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat cerebellum using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 18075-1-AP (PCDHA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 18075-1-AP (PCDHA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18075-1-AP (PCDHA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18075-1-AP (PCDHA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 18075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PCDHA9 antibody (18075-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

18075-1-AP bindet in WB, IHC, IF-P, IP, ELISA PCDHA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCDHA9 fusion protein Ag12767
• Vollständiger Name: protocadherin alpha 9
• Berechnetes Molekulargewicht: 950 aa, 102 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC104802
• Gene symbol: PCDHA9
• Gene ID (NCBI): 9752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA9 belongs to the α-protocadherin (PCDHA) cluster. A homozygous variant in PCDHA9 has been found in three unrelated Chinese ALS patients, suggesting PCDHA9 as a candidate gene for amyotrophic lateral sclerosis(PMID: 38467605).

Publikationen

Species	Application	Title
human	WB	Gene; Identification of two novel PCDHA9 mutations associated with Hirschsprung's disease.; Authors - Qiyang Shen ; KD Validated
human,mouse	WB,IF	Nat Commun; PCDHA9 as a candidate gene for amyotrophic lateral sclerosis; Authors - Jie Zhong