PCK1 Monoklonaler Antikörper

PCK1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11B5

Kat-Nr. : 66862-1-PBS

Synonyme

PCK1, PEPCK C, PEPCK1, PEPCKC

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  WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66862-1-PBS bindet in WB, IHC, IF/ICC, ELISA PCK1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PCK1 fusion protein Ag9720
• Vollständiger Name: phosphoenolpyruvate carboxykinase 1 (soluble)
• Berechnetes Molekulargewicht: 622 aa, 69 kDa
• Beobachtetes Molekulargewicht: 65-69 kDa
• GenBank-Zugangsnummer: BC023978
• Gene symbol: PCK1
• Gene ID (NCBI): 5105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).