PCK1/2 Polyklonaler Antikörper

PCK1/2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14892-1-PBS

Synonyme

PCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2

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  WB analysis of mouse brain using 14892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14892-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14892-1-PBS bindet in WB, IHC, IF/ICC, ELISA PCK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCK1/2 fusion protein Ag6681
• Vollständiger Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 66-71 kDa
• GenBank-Zugangsnummer: BC001454
• Gene symbol: PCK2
• Gene ID (NCBI): 5106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.