PCK2 Monoklonaler Antikörper

PCK2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3F1

Kat-Nr. : 67676-1-PBS

Synonyme

1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

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  WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67676-1-Ig (same clone as 67676-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

  IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCK2 antibody (67676-1-Ig, Clone: 1B3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67676-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA PCK2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PCK2 fusion protein Ag7021
• Vollständiger Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC001454
• Gene symbol: PCK2
• Gene ID (NCBI): 5106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.