PCM1 Rekombinanter Antikörper

PCM1 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242199D6

Kat-Nr. : 84979-2-PBS

Synonyme

pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1

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  IHC staining of mouse heart using 84979-2-RR (same clone as 84979-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84979-2-RR (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84979-2-RR (same clone as 84979-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84979-2-RR (PCM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84979-2-RR (same clone as 84979-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84979-2-RR (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84979-2-RR (same clone as 84979-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-2-RR, Clone: 242199D6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84979-2-RR (same clone as 84979-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (84979-2-RR, Clone: 242199D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 84979-2-RR (same clone as 84979-2-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-2-RR, Clone:242199D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84979-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84979-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84979-2-RR against Human PCM1 were performed. The affinity constant is 3.24 nM.

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Geprüfte Anwendungen

Produktinformation

84979-2-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA PCM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PCM1 fusion protein Ag13977
• Vollständiger Name: pericentriolar material 1
• Berechnetes Molekulargewicht: 2024 aa, 228 kDa
• GenBank-Zugangsnummer: BC000453
• Gene symbol: PCM1
• Gene ID (NCBI): 5108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).