PCMT1 Polyklonaler Antikörper

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• KD/KO Validated

PCMT1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 10519-1-AP

Synonyme

L-isoaspartyl protein carboxyl methyltransferase, PCMT-1, PIMT, protein L isoaspartate (D aspartate) O methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase

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  WB analysis using 10519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10519-1-AP

  WB result of PCMT1 antibody (10519-1-AP, 1:1000) with si-Control and si-PCMT1 transfected HeLa cells.

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  WB analysis of HeLa using 10519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10519-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10519-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa, HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, Maushodengewebe, Raji-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

10519-1-AP bindet in WB, IHC, IF/ICC, ELISA PCMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCMT1 fusion protein Ag0811
• Vollständiger Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC008748
• Gene symbol: PCMT1
• Gene ID (NCBI): 5110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Publikationen

Species	Application	Title
human	WB	Diabetologia; Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?; Authors - W?gner A M AM
mouse	WB	Int J Cardiol; Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.; Authors - Yan Guijun G
human	WB	Front Cell Dev Biol; LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.; Authors - Li-Ming Dong
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	IHC	Transl Cancer Res; Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer; Authors - Liyun Shan
human	WB	J Exp Clin Cancer Res; Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.; Authors - Jingjing Zhang ; KD Validated

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