PCMTD1 Polyklonaler Antikörper

PCMTD1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24425-1-AP

Synonyme

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1, Protein L isoaspartate O methyltransferase domain containing protein 1

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  WB analysis using 24425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24425-1-AP (PCMTD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 24425-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCMTD1 antibody (24425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, hTERT-RPE1-Zellen, humanes Plazenta-Gewebe, Maus-Augengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24425-1-AP bindet in WB, IF/ICC, ELISA PCMTD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCMTD1 fusion protein Ag19634
• Vollständiger Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1
• Berechnetes Molekulargewicht: 357 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa, 27 kDa
• GenBank-Zugangsnummer: BC032670
• Gene symbol: PCMTD1
• Gene ID (NCBI): 115294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein-l-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1) is a putative E3 ubiquitin ligase substrate adaptor protein, which has a C-terminal domain containing SOCS-box ubiquitin ligase recruitment motifs. PCMTD1 protein may provide an alternate maintenance pathway for modified proteins in mammalian cells (PMID: 35486881). This antibody can detect two isoforms of PCMTD1, 27 kDa and 41 kDa respectively (PMID: 37953789, 35486881).