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PCNA Polyklonaler Antikörper
PCNA Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IP, IHC, IF, CoIP, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10205-2-AP
Synonyme
"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, C2C12-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Mauslebergewebe, Mausmilzgewebe, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Raji-Zellen, Rattenhodengewebe, Rattenlebergewebe |
Erfolgreiche IP | MCF-7-Zellen, N/A |
Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes malignes Melanomgewebe, humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF | HepG2-Zellen, humanes Mammakarzinomgewebe, Maushodengewebe, MCF-7-Zellen, Neuro-2a-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1500-1:6000 |
Immunfluoreszenz (IF) | IF : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 597 publications below |
IHC | See 217 publications below |
IF | See 74 publications below |
ELISA | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
10205-2-AP bindet in WB, IP, IHC, IF, CoIP, Cell treatment, ELISA PCNA und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Fisch, Huhn, Kaninchen, Maus, Ratte, Ziege, Medaka-Embryos |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | PCNA fusion protein Ag0277 |
Vollständiger Name | proliferating cell nuclear antigen |
Berechnetes Molekulargewicht | 29 kDa/31 kDa |
Beobachtetes Molekulargewicht | 36-38 kDa |
GenBank-Zugangsnummer | BC000491 |
Gene symbol | PCNA |
Gene ID (NCBI) | 5111 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for PCNA antibody 10205-2-AP | Protokoll herunterladen |
IHC protocol for PCNA antibody 10205-2-AP | Protokoll herunterladen |
IF protocol for PCNA antibody 10205-2-AP | Protokoll herunterladen |
IP protocol for PCNA antibody 10205-2-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Mil Med Res Aspartoacylase suppresses prostate cancer progression by blocking LYN activation | ||
Brain Behav Immun An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling. | ||
Nat Commun The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer | ||
Nat Commun Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction | ||
Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
Hepatology Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mi (Verified Customer) (06-25-2023) | It works well in human brown adipocyte cells.
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FH Emma (Verified Customer) (11-29-2021) | Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50
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