PCNA Polyklonaler Antikörper

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• KD/KO Validated

PCNA Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF-P, IP, CoIP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(1096)

Rezensionen (2)

Kat-Nr. : 10205-2-AP

Synonyme

Cyclin, proliferating cell nuclear antigen

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of HEK293 cells using 10205-2-AP

  WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10205-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 10205-2-AP

  IP result of anti-PCNA (IP:10205-2-AP, 4ug; Detection:10205-2-AP 1:20000) with MCF-7 cells lysate 1880 ug.

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  IP experiment using 10205-2-AP

  IP result of anti-PCNA (10205-2-AP for IP and Detection) with MCF7 cell.

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  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10205-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human breast cancer using 10205-2-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10205-2-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C2C12-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausmilzgewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes malignes Melanomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe, humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1500-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 812 publications below
• IHC: See 283 publications below
• IF: See 103 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10205-2-AP bindet in WB, IHC, IF-P, IP, CoIP, ELISA, Cell treatment PCNA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Huhn, Kaninchen, Maus, Ratte, Ziege, Medaka-Embryos
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCNA fusion protein Ag0277
• Vollständiger Name: proliferating cell nuclear antigen
• Berechnetes Molekulargewicht: 29 kDa/31 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC000491
• Gene symbol: PCNA
• Gene ID (NCBI): 5111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Publikationen

Species	Application	Title
	WB	J Hematol Oncol; METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation; Authors - Meilin Xue
human	WB	Mil Med Res; Aspartoacylase suppresses prostate cancer progression by blocking LYN activation; Authors - Hong Weng
mouse,human	WB	Protein Cell; NDFIP1 limits cellular TAZ accumulation via exosomal sorting to inhibit NSCLC proliferation; Authors - Yirui Cheng
mouse	IF	Brain Behav Immun; An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signaling.; Authors - Fangfang Qi
rabbit	IF	Nat Commun; Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction; Authors - Yujie Hua
human	WB	Nat Commun; The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer; Authors - Wei Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

It works well in human brown adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human brown adipocytes

FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well on PC3 cells fixed with 4% PFA. Antibody used at 1:50

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PC3 cells

PCNA Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:10205-2-AP)

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