PCNA Polyklonaler Antikörper

PCNA Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(37)

Kat-Nr. : 24036-1-AP

Synonyme

Cyclin, proliferating cell nuclear antigen

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  WB analysis of HEK-293 using 24036-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24036-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24036-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24036-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 24036-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24036-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24036-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24036-1-AP

  IP result of anti-PCNA (IP:24036-1-AP, 4ug; Detection:24036-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human gliomas using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human gliomas using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 24036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human breast cancer using 24036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushodengewebe, NIH/3T3-Zellen, Raji-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 16 publications below
• IF: See 9 publications below
• RIP: See 1 publications below

Produktinformation

24036-1-AP bindet in WB, IHC, IF-P, IP, RIP, ELISA PCNA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCNA fusion protein Ag7416
• Vollständiger Name: proliferating cell nuclear antigen
• Berechnetes Molekulargewicht: 29 kDa/31 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC000491
• Gene symbol: PCNA
• Gene ID (NCBI): 5111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.This antibody specifically react with the 36kd human PCNA protein.

Publikationen

Species	Application	Title
mouse	IHC	Int J Biol Sci; Tendon Cells Root Into (Instead of Attach to) Humeral Bone Head via Fibrocartilage-Enthesis; Authors - Zheng Wang
rat	WB,IF	Biochem Pharmacol; Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo to Trigger Phenotypic Switching of Pulmonary Artery Smooth Muscle Cells; Authors - Ning Huang
rat	WB	FASEB J; S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.; Authors - Chao Fang
rat	WB,IHC	Biochem Pharmacol; CGRP attenuates pulmonary vascular remodeling by inhibiting the cGAS-STING-NFκB pathway in pulmonary arterial hypertension; Authors - Xin Yan
human	WB	Am J Cancer Res; YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis; Authors - Ya-Ni Chen
mouse	WB	Hepatol Commun; Hepatocellular carcinoma cells downregulate NADH:Ubiquinone Oxidoreductase Subunit B3 to maintain reactive oxygen species homeostasis; Authors - Zhendong Zhang