PCP4L1 Polyklonaler Antikörper

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• KD/KO Validated

PCP4L1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25933-1-AP

Synonyme

IQM1, PCP4 like protein 1, PCP4L1, Purkinje cell protein 4 like 1

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  WB analysis of mouse brain using 25933-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25933-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

25933-1-AP bindet in WB, IHC, ELISA PCP4L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCP4L1 fusion protein Ag23061
• Vollständiger Name: Purkinje cell protein 4 like 1
• Berechnetes Molekulargewicht: 68 aa, 7 kDa
• Beobachtetes Molekulargewicht: 8-15 kDa
• GenBank-Zugangsnummer: BC028905
• Gene symbol: PCP4L1
• Gene ID (NCBI): 654790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

Publikationen

Species	Application	Title
mouse	WB	Genome Biol; A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation.; Authors - Baokun Sui ; KD Validated
mouse	IHC	J Gen Virol; lncRNA EDAL restricts rabies lyssavirus replication in a cell-specific and infection route-dependent manner.; Authors - Baokun Sui
mouse	WB	J Proteome Res; Multiplexed Quantitative Proteomics Reveals Proteomic Alterations in Two Rodent Traumatic Brain Injury Models; Authors - Junho Park