PCSK1 Polyklonaler Antikörper

PCSK1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28219-1-AP

Synonyme

BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3

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  WB analysis using 28219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, Maushirngewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

28219-1-AP bindet in WB, IHC, ELISA PCSK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCSK1 fusion protein Ag28203
• Vollständiger Name: proprotein convertase subtilisin/kexin type 1
• Berechnetes Molekulargewicht: 753 aa, 84 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC130295
• Gene symbol: PCSK1
• Gene ID (NCBI): 5122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

Publikationen

Species	Application	Title
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
human	IHC	bioRxiv; Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue; Authors - Su Min Hong
human	IHC	Rapid Commun Mass Spectrom; Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm; Authors - Meng Zhang