PCSK1 Polyklonaler Antikörper

PCSK1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28219-1-PBS

Synonyme

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  WB analysis using 28219-1-AP (same clone as 28219-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28219-1-AP (same clone as 28219-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28219-1-PBS bindet in WB, IHC, Indirect ELISA PCSK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCSK1 fusion protein Ag28203
• Vollständiger Name: proprotein convertase subtilisin/kexin type 1
• Berechnetes Molekulargewicht: 753 aa, 84 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC130295
• Gene symbol: PCSK1
• Gene ID (NCBI): 5122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.