PCSK2 Polyklonaler Antikörper

PCSK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 10553-1-AP

Synonyme

EC:3.4.21.94, KEX2-like endoprotease 2, NEC2, PC2, SPC2

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  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10553-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

10553-1-AP bindet in WB, IHC, IF, ELISA PCSK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCSK2 fusion protein Ag0852
• Vollständiger Name: proprotein convertase subtilisin/kexin type 2
• Berechnetes Molekulargewicht: 70.5 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC005815
• Gene symbol: PCSK2
• Gene ID (NCBI): 5126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)

Publikationen

Species	Application	Title
human	IF	Nat Commun; Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.; Authors - Chikafumi Ozone
rat	WB	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li
mouse	WB	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Function (Oxf); ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell; Authors - Kristen E Rohli
mouse	IF	Front Mol Biosci; Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease; Authors - Fang Jia
		iScience; Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells; Authors - Mingchao Jiang

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