PCSK2 Polyklonaler Antikörper

PCSK2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10553-1-PBS

Synonyme

EC:3.4.21.94, KEX2-like endoprotease 2, NEC2, PC2, SPC2

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  WB analysis using 10553-1-AP (same clone as 10553-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

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  WB analysis using 10553-1-AP (same clone as 10553-1-PBS)

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10553-1-AP (same clone as 10553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 10553-1-AP (same clone as 10553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10553-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10553-1-PBS bindet in WB, IHC, Indirect ELISA PCSK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCSK2 fusion protein Ag0852
• Vollständiger Name: proprotein convertase subtilisin/kexin type 2
• Berechnetes Molekulargewicht: 70.5 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC005815
• Gene symbol: PCSK2
• Gene ID (NCBI): 5126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)

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