PCSK9 Rekombinanter Antikörper

PCSK9 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241395B7

Kat-Nr. : 84172-6-PBS

Synonyme

EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1, NARC 1

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  Sandwich ELISA standard curve of MP01071-4

  Sandwich ELISA standard curve of MP01071-4, Human PCSK9 Recombinant Matched Antibody Pair - PBS only. 84172-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0362. 84172-6-PBS was HRP conjugated as the detection antibody. Range: 195-6250 pg/mL.

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  Sample test of MP01071-4

  Plasma of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 206.0 ng/mL with a range of ND-514.5 ng/mL.

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  Sample test of MP01071-4

  HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human PCSK9, and measured 81.6 ng/mL.

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  Sample test of MP01071-4

  The mean PCSK9 concentration was determined to be 6.6 ng/mL in HepG2 cell extract based on a 2.0 mg/mL extract load.

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  Sample test of MP01071-4

  Serum of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 120.8 ng/mL with a range of ND-421.1 ng/mL.

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  Affinity and Kinetic Characterization of 84172-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 84172-6-PBS against Human PCSK9 was performed. The affinity constant is 2.55 nM.

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Geprüfte Anwendungen

Produktinformation

84172-6-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test PCSK9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PCSK9 fusion protein Eg0362
• Vollständiger Name: proprotein convertase subtilisin/kexin type 9
• Berechnetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: NM_174936.4
• Gene symbol: PCSK9
• Gene ID (NCBI): 255738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.