PCYT1A Polyklonaler Antikörper

PCYT1A Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30991-1-AP

Synonyme

CCT A, CCT alpha, CT A, CTPCT, PCYT1

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  WB analysis using 30991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U-251 using 30991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen, Maushodengewebe, Rattenhodengewebe, Y79-Zellen
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30991-1-AP bindet in WB, IF/ICC, ELISA PCYT1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCYT1A fusion protein Ag34268
• Vollständiger Name: phosphate cytidylyltransferase 1, choline, alpha
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 40-41 kDa
• GenBank-Zugangsnummer: NM_005017
• Gene symbol: PCYT1A
• Gene ID (NCBI): 5130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.