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PCYT1A Polyklonaler Antikörper

PCYT1A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30991-1-PBS

Synonyme

CCT A, CCT alpha, CT A, CTPCT, PCYT1

Filter:
  • Western Blot (WB) analysis of various lysates using PCYT1A Polyclonal antibody (30991-1-AP)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCYT1A Polyclonal antibody (30991-1-AP)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PCYT1A Polyclonal antibody (30991-1-AP)

    IF Staining of A431 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PCYT1A Polyclonal antibody (30991-1-AP)

    IF Staining of U-251 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30991-1-PBS bindet in WB, IF/ICC, Indirect ELISA PCYT1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PCYT1A fusion protein Ag34268
Vollständiger Name phosphate cytidylyltransferase 1, choline, alpha
Berechnetes Molekulargewicht42 kDa
Beobachtetes Molekulargewicht40-41 kDa
GenBank-ZugangsnummerNM_005017
Gene symbol PCYT1A
Gene ID (NCBI) 5130
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.