PCYT1A Rekombinanter Antikörper

PCYT1A Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230393B4

Kat-Nr. : 83100-1-PBS

Synonyme

230393B4, CCT A, CCT alpha, CT A, CTPCT

---

• 

  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 83100-1-RR (same clone as 83100-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83100-1-RR (same clone as 83100-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83100-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

83100-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA PCYT1A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PCYT1A fusion protein Ag34268
• Vollständiger Name: phosphate cytidylyltransferase 1, choline, alpha
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 38-41 kDa
• GenBank-Zugangsnummer: NM_005017
• Gene symbol: PCYT1A
• Gene ID (NCBI): 5130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.