PCYT1B/PCYT1A Polyklonaler Antikörper

PCYT1B/PCYT1A Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13765-1-AP

Synonyme

PCYT1B, CCT B, CCT beta, CCTB, CCT-beta

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  WB analysis using 13765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13765-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 13765-1-AP

  IP result of anti-PCYT1B (IP:13765-1-AP, 4ug; Detection:13765-1-AP 1:500) with human placenta tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, humanes Hirngewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13765-1-AP bindet in WB, IP, ELISA PCYT1B/PCYT1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCYT1B/PCYT1A fusion protein Ag4748
• Vollständiger Name: phosphate cytidylyltransferase 1, choline, beta
• Berechnetes Molekulargewicht: 351 aa, 40 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC045634
• Gene symbol: PCYT1B
• Gene ID (NCBI): 9468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PCYT1B (Phosphate Cytidylyltransferase 1B, Choline), also named as CTβ, CCTB and CCT-Beta, is encoded by the gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. PCYT1B encodes three isoforms of CTβ (CTβ1, CTβ2 and CTβ3). CTβ1, CTβ2 isoforms are present in humans and CTβ2 and CTβ3 isoforms have been identified in mice. The CTβ2 can be detected as 41 kDa, and CTβ3 can be detected as 39 kDa. This antibody can recognizes both PCYT1A and PCYT1B. PCYT1A usually exists in the form of a 80 kDa dimer.

Publikationen

Species	Application	Title
mouse	WB	Neurobiol Dis; Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis; Authors - Fan Zhang