PCYT2 Polyklonaler Antikörper

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• KD/KO Validated

PCYT2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14827-1-AP

Synonyme

ET, PCYT2

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Galerie der Validierungsdaten

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  WB analysis of human liver using 14827-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14827-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14827-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14827-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 14827-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14827-1-AP (PCYT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HepG2-Zellen, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14827-1-AP bindet in WB, IHC, IF, ELISA PCYT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PCYT2 fusion protein Ag6616
• Vollständiger Name: phosphate cytidylyltransferase 2, ethanolamine
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000351
• Gene symbol: PCYT2
• Gene ID (NCBI): 5833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

Publikationen

Species	Application	Title
mouse	WB	Nature; Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine.; Authors - Guotong Fu ; KO Validated
human	IHC	Int J Mol Sci; Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan.; Authors - Fumiko Umezawa
human,mouse	IF,WB	Int J Biol Macromol; p75NTR enhances cognitive dysfunction in a mouse Alzheimer's disease model by inhibiting microRNA-210-3p-mediated PCYT2 through activation of NF-κB; Authors - Zhongliang Wei ; KD Validated
human	WB	Evid Based Complement Alternat Med; Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.; Authors - Shanshan Nie