PD-1/CD279 Rekombinanter Antikörper

PD-1/CD279 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240390D5

Kat-Nr. : 83516-4-PBS

Synonyme

CD279, PD1, PD-1, PD 1, Pdc1

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  Sandwich ELISA standard curve of MP00496-4

  Sandwich ELISA standard curve of MP00496-4, Mouse PD-1/CD279 Monoclonal Matched Antibody Pair - PBS only. 83516-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0918. 83516-9-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP00496-4

  The mean mouse PD-1/CD279 concentration was determined to be 14.08 ng/mL in EL-4 cell extract based on a 3.0 mg/mL extract load.

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  Sample test of MP00496-4

  EL-4 was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL ofstreptomycin sulfate.The cellswere stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant wasremoved, assayed for mouse PD-1/CD279 and measured 812.8 pg/mL.

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Geprüfte Anwendungen

Produktinformation

83516-4-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PD-1/CD279 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Eg0918
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_008798.2
• Gene symbol: PD-1
• Gene ID (NCBI): 18566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.