PD-1/CD279 Rekombinanter Antikörper

PD-1/CD279 Rekombinant Antikörper für FC, Blocking

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC, Blocking

Konjugation

Unkonjugiert

CloneNo.

240389B8

Kat-Nr. : 98080-1-RR

Synonyme

CD279, PD1, PD-1, 240389B8, PD 1

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  FC experiment of mouse splenocytes using 98080-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit RecAb (98080-1-RR, Clone: 240389B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of mouse splenocytes using 98080-1-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit RecAb (98080-1-RR, Clone: 240389B8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1 Rabbit Recombinant Antibody (98080-1-RR, Clone: 240389B8) (black, dashed). Cells were not fixed.

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  Blocking experiment of Recombinant protein using 98080-1-RR

  Inhibition assay of CD279 using 98080-1-RR by ELISA. Mouse PD-1 (Eg0986) was coated at 5 ug/mL. Serial diluted 98080-1-RR and 5 ug/ml biotin-labelled mouse PD-L1 was applied, and the binding signal of PD-L1 to PD-1 was detected by HRP-SA. Signal was developed by TMB substrate. The IC50 is 0.03849 ug/ml.

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  Affinity and Kinetic Characterization of 98080-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98080-1-RR against Mouse PD-1/CD279 were performed. The affinity constant is 5.21 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: anti-CD3/CD28 treated mouse splenocytes
• Erfolgreiche Detektion in Blocking: Recombinant protein

Empfohlene Verdünnung

• BLOCKING: BLOCKING :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

98080-1-RR bindet in FC, Blocking PD-1/CD279 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Eg0918
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_008798.2
• Gene symbol: PD-1
• Gene ID (NCBI): 18566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).