PD-1/CD279 Rekombinanter Antikörper

PD-1/CD279 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

29F.1A12

Kat-Nr. : 98080-6-MR

Synonyme

CD279, PD1, PD-1, PD 1, Pdc1

---

• 

  FC experiment of mouse splenocytes using 98080-6-MR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Mouse RecAb (98080-6-MR, Clone: 29F.1A12) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98080-6-MR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Mouse RecAb (98080-6-MR, Clone: 29F.1A12) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG2a. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Mouse RecAb (98080-6-MR, Clone: 29F.1A12) (black, dashed) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98080-6-MR bindet in FC PD-1/CD279 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: unknow
• Vollständiger Name: programmed cell death 1
• Gene symbol: PD-1
• Gene ID (NCBI): 18566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706).