PD-1/CD279 Monoklonaler Antikörper

PD-1/CD279 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

J110

Kat-Nr. : CL594-65119

Synonyme

CD279, PD1, PD-1, PD 1, J110

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  FC experiment of human PBMCs using CL594-65119

  1x10^6 PHA treated (3 day) human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone: J110) (red) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone:J110) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using CL594-65119

  1x10^6 PHA treated (3 day) human PBMCs were surface stained with CL405 Anti-Human CD3 (CL405-65151, Clone: UCHT1) and 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone: J110) (right) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21) (left). 1x10^6 untreated human PBMCs were surface stained with CL405 Anti-Human CD3 (CL405-65151, Clone:UCHT1) and 5 ul CoraLite® Plus 594 Anti-Human PD-1/CD279 (CL594-65119, Clone:J110) (center). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65119 bindet in FC PD-1/CD279 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PD-1
• Gene ID (NCBI): 5133
• Konjugation: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 594 nm / 615 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).