PD-1/CD279 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PD-1/CD279 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4H4D1

Kat-Nr. : CL594-66220

Synonyme

CD279, PD1, PD-1, 4H4D1, PD 1

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Lymphomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66220 bindet in IF-P PD-1/CD279 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 47-55 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PD-1
• Gene ID (NCBI): 5133
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).