PD-1/CD279 Monoklonaler Antikörper

PD-1/CD279 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen

2

CloneNo.

4H4D1

Kat-Nr. : CL647-66220

Synonyme

CD279, hPD 1, hPD l, PD1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL647-66220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL647-66220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:100.

• 

  IF Staining of human tonsillitis using CL647-66220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 647 PD-1/CD279 antibody (CL647-66220, Clone: 4H4D1 ) at dilution of 1:200, CoraLite®594 CD11c/Integrin Alpha X antibody (CL594-60258, Clone: 2F1C10, red), CoraLite®488 CD8 antibody (CL488-66868, Clone: 1G2B10, green).

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  FC experiment of PBMC using CL647-66220

  1X10^6 PHA-stimulated (5 μg/mL, overnight) human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.20 ug CoraLite647-conjugated Anti-Human PD-1/CD279 (CL647-66220, clone: 4H4D1) and 0.20 ug FITC-Anti-Human CD3 (FITC-65151, clone UCHT1). Cells were not fixed.

• 

  FC experiment of PBMC using CL647-66220

  1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with 0.20 ug CoraLite647-conjugated Anti-Human PD-1/CD279 (CL647-66220, clone: 4H4D1) and 0.20 ug FITC-Anti-Human CD3 (FITC-65151, clone UCHT1). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: PBMC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

CL647-66220 bindet in IF, FC PD-1/CD279 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PDCD1
• Gene ID (NCBI): 5133
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

Publikationen

Species	Application	Title
mouse	IF	Clin Cancer Res; 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition.; Authors - Kirsten M Reeves
rat	FC	Transplantation; Activation of the Aryl Hydrocarbon Receptor Ameliorates Acute Rejection of Rat Liver Transplantation by Regulating Treg Proliferation and PD-1 Expression.; Authors - Wanyue Cao