PD-1/CD279 Rekombinanter Antikörper

PD-1/CD279 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

240389B8

Kat-Nr. : FITC-98080

Synonyme

CD279, PD1, PD-1, 240389B8, PD 1

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  FC experiment of mouse splenocytes using FITC-98080

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with APC Anti-Mouse CD3 and 0.25 ug FITC Plus Anti-Mouse PD-1/CD279 Rabbit RecAb (FITC-98080, Clone: 240389B8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-98080

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse PD-1/CD279 Rabbit RecAb (FITC-98080, Clone: 240389B8) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse PD-1/CD279 Rabbit RecAb (FITC-98080, Clone: 240389B8) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98080 bindet in FC PD-1/CD279 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Eg0918
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_008798.2
• Gene symbol: PD-1
• Gene ID (NCBI): 18566
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).